Lidiia Bondareva

F.F. Erisman, Moscow Region, Russia

Abstract Title: The Use of Radioautography for Investigating Wall Secretion in Plant Cells of Tea (leaves)

Biography:

Lidiia Bondareva is PhD of Analytical Chemistry and Full Professor. She is a leading researcher at the Department of Analytical Methods for Researching Food Products and Environmental Objects. She is leading researcher in field behavior pesticides in environment and residue in food products. She has published more than 200 scientific articles in renowned scientific journals. She is the author of 10 monographs and 7 patents.

Research Interest:

The control of tea diseases and pests mainly relies on chemical methods, supplemented by agricultural and biological approaches. This study provides an assessment of the migration behavior pesticide in tea, when we used radiolabel pesticide. Method – Radioautography and liquid scintillation spectrometry. Plants - the tea plant (Azorean Camellia sinensis) - leaves from different levels of the plant. Pesticide – pyraclostrobin-14C (15 kBq/m2). Soil - primarily fertile, acidic, volcanic soils with good drainage, rich in organic matter (layers 0-10 cm, 10-20 cm, 20-30 cm). Experiment – radiolabeled pesticides like solutions: 1) with surfactants and 2) without surfactants, were added in soil around plant. In preliminary experiments, excised leaves were collected for periods between ~ 24 days. With longer incubations (60 days) massive labelling of the walls of all leaves at the bottom of the bush cap cells was detected. While for leaves for levels above the ground - it's less than 10 %. The walls of more internally situated cells of the leaves tip differ in some respect to those of epidermal cells; this difference is apparent in permanganate-fixed material viewed with the electron microscope, since the walls of the latter cells stain heavily, in contrast to the walls of the internal cells. Distribution between layers was: 0-10 cm ⁓ 40 % activity, 10-20 cm ⁓ 30 % activity and 20-30 cm ⁓ 10 % activity from total activity of 14C. For plants, we didn’t have significant difference between using the pyraclostrobin-14C with or without surfactants.